THE AMINO ACID COMPOSITION OF CRYSTALLINE PEPSIN
نویسندگان
چکیده
منابع مشابه
The Amino Acid Composition of Crystalline Pepsin
The amino acid composition of twice recrystallized pepsin (Worthington Biochemical Corporation) has been determined chromatographically on columns of Amberlite IR 120 resin. The results of the analyses obtained on four different preparations indicate a close agreement in their amino acid composition. Pepsin is unique in that it has a great predominance of acidic amino acids over basic ones. Mor...
متن کاملCrystalline Pepsin
The decrease in protein nitrogen and in the activity of solutions of crystalline pepsin at pH 1.8 and 45 degrees C. has been determined. The decrease in activity, as measured with eleven different methods, is in exact proportion to the decrease of protein nitrogen of the solution. The measurements were continued until less than 5 per cent of the original protein remained. These results indicate...
متن کاملAmino-acid sequence of porcine pepsin.
As the culmination of several years of experiments, we propose a complete amino-acid sequence for porcine pepsin, an enzyme containing 327 amino-acid residues in a single polypeptide chain. In the sequence determination, the enzyme was treated with cyanogen bromide. Five resulting fragments were purified. The amino-acid sequence of four of the fragments accounted for 290 residues. Because the s...
متن کاملCrystalline Pepsin Iii. Preparation of Active Crystalline Pepsin from Inactive Denatured Pepsin By
The results (1) of the experiments with crystalline pepsin isolated from crude pepsin preparations indicated that the material is a pure substance and that the proteolytic activity is a property of the protein molecule itself and is not due to the presence of a separate non-protein impurity. No indication of the presence of a more highly active nonprotein molecule was obtained in the solubility...
متن کاملThe amino acid sequence at the N terminus of pepsin.
Attempts to apply the technique of Edman (l), for the stepwise degradation of peptides with the formation of phenylthiohydantoins (PTH), to the study of the N-terminal amino acid sequence of pepsin have resulted in only qualified success. One of the principal drawbacks of this technique arises from the fact that the reactions entailed are usually not complete. Thus, residual amounts of terminal...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1959
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.42.3.553